Two methods for measuring carbohydrate-deficient transferrin in inpatient alcoholics and healthy controls compared.

Nephelometric determination of carbohydrate deficient transferrin.

We describe a technique for measuring carbohydrate-deficient transferrin (CDT) in serum. Serum transferrin fractions are separated by anion-exchange chromatography on microcolumns. Sialic acid-deficient transferrin fractions are collected in the eluate, and transferrin is then quantified by a rate-nephelometric technique. Imprecision (CV) was 4-5% within-run and 7-9% between runs (n = 15). Comp...

Interference of transferrin isoform types with carbohydrate-deficient transferrin quantification in the identification of alcohol abuse.

BACKGROUND Isoforms of transferrin interfere with measurement of carbohydrate-deficient transferrin (CDT) as a marker of heavy alcohol consumption. We evaluated the rate of inaccurate CDT results by immunoassays. METHODS We studied 2360 consecutive sera (1614 individuals) submitted for CDT assay without clinical information as well as samples from 1 patient with a congenital disorder of glyco...

Improved HPLC method for carbohydrate-deficient transferrin in serum.

BACKGROUND There is need for a reference method for transferrin glycoforms in serum to which routine immunologic methods for the alcohol marker carbohydrate-deficient transferrin (CDT) can be traceable. We describe an improved HPLC method for transferrin glycoforms. METHODS Transferrin was iron-saturated by mixing the serum with ferric nitrilotriacetic acid, and lipoproteins were precipitated...

Carbohydrate-deficient transferrin in patients with alcoholic cardiomyopathy

Erythrocyte riboflavin for the detection of riboflavin deficiency in pregnant Nepali women.

chronic alcohol abuse in drivers. Forensic Sci Int;in press. 6. Berkowicz A, Wallerstedt S, Wall K, Denison H. Carbohydrate-deficient transferrin in vitreous humour: a marker of possible withdrawal-related death in alcoholics. Alcohol 2001;36:231–4. 7. Helander A, Eriksson G, Stibler H, Jeppsson J-O. Interference of transferrin isoform types with carbohydrate-deficient transferrin quantificatio...